主講人📠📫：Dr. Weerawat Runguphan              

報告時間✔️📓：2019-06-11  上午10🤾🏻‍♀️📆：00-11🈶：00

地點🔱：EON体育4平台  3-105

聯系人🦤：趙心清   xqzhao@sjtu.edu.cn

報告人簡介🚁：

Runguphan 博士2006年在哈佛大學獲得Chemistry and Physics學士學位(Magna Cum Laude with Highest Honors)，2011年在MIT獲得生物化學博士學位，2011-1014年在美國Lawrence Berkeley National Lab從事合成生物學相關博士後研究工作🏊🏽‍♀️，合作導師Jay Keasling教授。2014年起在泰國國家遺傳工程與生物技術研究所工作，任課題組長。主要從事釀酒酵母代謝工程改造和生物燃料生產相關研究。

講座簡介：

Interests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive alternative to the traditional production of transportation fuels. Here, we highlight two examples of engineering yeast systems to produce biofuels. In the first example, we engineered the budding yeast Saccharomyces cerevisiae to produce fatty acid-derived biofuels and chemicals from simple sugars. Our engineered strains were able to produce free fatty acids at a titer of approximately 400mg/L, fatty alcohols at approximately 100 mg/L and fatty acid ethyl esters (biodiesel) at approximately 5 mg/L directly from simple sugars. We further optimized our original production strains by increasing the cytosolic pool of NADPH, a redox cofactor that is essential in fatty acid biosynthesis. In the second example, we engineered Pichia pastoris, an industrial workhorse in heterologous enzyme production, to produce the biofuels isobutanol and isopentanol from two renewable carbon sources from renewable carbon sources. Our strategy exploited the yeast’s amino acid biosynthetic pathway and diverted the amino acid intermediates to the 2-keto acid degradation pathway for higher alcohol production. Rewiring of the cells' primary metabolism using CRISPR/Cas9 further improved product yields. Efforts are underway to create a consolidated bioprocessing platform based on a single microorganism that directly converts sugarcane bagasse, one of the most abundant agricultural wastes in Thailand, into advanced biofuels. We envision that our CBP platform will enable efficient utilization of agricultural wastes and provide an economic route to production of advanced biofuels and chemicals.